Nuclease Methods and Protocols

Abstract

Scientists at the forefront of academic research and pharmaceutical drug development offer their well-polished methodologies for protein nucleases that can be used to determine their activity, structure, interaction with other molecules, and physiological role. Protocols range from methods for characterizing nucleases, to correlating nuclease structure and function, to the use of nucleases in cloning. Recombinant techniques for expressing and isolating nucleases are covered, as well as methods specific for dealing with protein/nucleic acid complexes. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assay in question, and an explanation of the thinking that went into its development. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of their importance in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics.