A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding

dc.contributor.author	Vartia, Salla	en_US
dc.contributor.author	Villanueva-Cañas, José L.	en_US
dc.contributor.author	Finarelli, John	en_US
dc.date.accessioned	2016-10-11T05:37:22Z
dc.date.available	2016-10-11T05:37:22Z
dc.date.issued	2016	en_US
dc.identifier.other	HPU4160686	en_US
dc.identifier.uri	https://lib.hpu.edu.vn/handle/123456789/23578	en_US
dc.description.abstract	This study examines the potential of next-generation sequencing based ‘genotyping-by-sequencing’ (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture.	en_US
dc.format.extent	9 p.	en_US
dc.format.mimetype	application/pdf	en_US
dc.language.iso	en	en_US
dc.subject	Genetics	en_US
dc.subject	Genomics	en_US
dc.subject	Ecology	en_US
dc.subject	Amplicon sequencing	en_US
dc.subject	Universal primer	en_US
dc.title	A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding	en_US
dc.type	Article	en_US
dc.size	439KB	en_US
dc.department	Education	en_US